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‘one-color microarray-based gene expression analysis’ protocol version 5.7  (Agilent technologies)


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    Structured Review

    Agilent technologies ‘one-color microarray-based gene expression analysis’ protocol version 5.7
    Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. <t>Microarray</t> data values represented here are shown in and .
    ‘One Color Microarray Based Gene Expression Analysis’ Protocol Version 5.7, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray-based+gene+expression+analysis+protocol/pmc03629124-192-1-9
    Average 90 stars, based on 1 article reviews
    ‘one-color microarray-based gene expression analysis’ protocol version 5.7 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development"

    Article Title: Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0062135

    Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .
    Figure Legend Snippet: Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .

    Techniques Used: Functional Assay, Microarray

    Related Articles

    Gene Expression:

    Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier
    Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Agilent Hybridization kit (One-Color Microarray-based Gene Expression Analysis Protocol). .. The slides were scanned with the Agilent SureScan Microarray Scanner System.

    Microarray:

    Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier
    Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Agilent Hybridization kit (One-Color Microarray-based Gene Expression Analysis Protocol). .. The slides were scanned with the Agilent SureScan Microarray Scanner System.

    Hybridization:

    Article Title: Neryl acetate, the major component of Corsican Helichrysum italicum essential oil, mediates its biological activities on skin barrier
    Article Snippet: The total RNA (100 ng) of each sample was reverse transcribed, amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). .. Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8X60K V2 Arrays (SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit, G4851B; 50,599 probes) using reagents supplied in the Agilent Hybridization kit (One-Color Microarray-based Gene Expression Analysis Protocol). .. The slides were scanned with the Agilent SureScan Microarray Scanner System.

    other:

    Article Title: The Mechanisms of Maize Resistance to Fusarium verticillioides by Comprehensive Analysis of RNA-seq Data
    Article Snippet: A 2-mg aliquot of total RNA was used to synthesize fluorophore-labeled cRNA using cyanine 3-CTP (One-color microarray-based gene expression analysis protocol, Version 5.5; Agilent Technologies).

    Article Title: SRD‐1 in AWA neurons is the receptor for female volatile sex pheromones in C. elegans males
    Article Snippet: Oligonucleotide microarrays were hybridized using an Agilent C. elegans Oligo 4 × 44 K Microarray (Agilent, USA) in an Agilent microarray hybridization chamber, following the protocol of Agilent Two‐color Microarray‐Based Gene Expression Analysis 60 .

    Article Title: Increased Skin Papilloma Formation in Mice Lacking Glutathione Transferase GSTP
    Article Snippet: Total RNA (1 mg) was labeled with Cyanine 3 (Cy3)-CTP (Agilent One-Colour Microarray-Based Gene Expression Analysis protocol, v5.0.1) using the Low Input RNA Fluorescent Linear Amplification Kit (Agilent).

    Article Title: Increased skin papilloma formation in mice lacking glutathione transferase GSTP
    Article Snippet: Total RNA (1μg) was labelled with Cyanine 3 (Cy3)-CTP (Agilent One-Colour Microarray-Based Gene Expression Analysis protocol, v5.0.1) using the Low Input RNA Fluorescent Linear Amplification Kit (Agilent).

    Article Title: miR-196b directly targets both HOXA9/MEIS1 oncogenes and FAS tumour suppressor in MLL -rearranged leukaemia
    Article Snippet: 0.5 μg total RNA per sample was reverse transcribed, amplified and labeled with Cy3 using Agilent one-color amplification protocol (Agilent Quick-Amp Labeling Kit, One-color; One-Color Microarray-Based Gene Expression Analysis).



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    Image Search Results


    Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .

    Journal: PLoS ONE

    Article Title: Defining the Genomic Signature of Totipotency and Pluripotency during Early Human Development

    doi: 10.1371/journal.pone.0062135

    Figure Lengend Snippet: Significant genes of each signature were represented according to their gene function and specific role in the cell, localization and type of interaction between them. Node border color refers to cell localization, node shape to general function and node color to specific function in the cell. Edge color refers to physical interactions, biochemical interactions or to both; when not specified, a functional interaction is assumed. Upstream arrow (red) means up-regulation versus the other categories, and downstream arrow (blue) means down-regulation versus the other categories. Microarray data values represented here are shown in and .

    Article Snippet: Following ‘One-Color Microarray-Based Gene Expression Analysis’ protocol Version 5.7 (Agilent p/n G4140-90040), 3 µg of labelled cRNA was hybridised with the Whole Human Genome Oligo Microarray Kit (Agilent p/n G2519F-014850) containing 41,000+ unique human genes and transcripts.

    Techniques: Functional Assay, Microarray

    Expression Profiles of differently expressed lncRNAs and mRNAs screened by microarray analysis of fibroblast-like synoviocytes (FLSs) samples from rheumatoid arthritis (RA) group ( n = 3) and control group ( n = 3). With the threshold of fold changes > 1.5 and P < 0.05, (a) the Volcano Plot showed differentially expressed lncRNAs between RA-FLSs and HC-FLSs and (b) the Scatter Plot showed differentially expressed mRNAs. (c) The Heat Map showing distinguishable lncRNA expression profiles with fold changes > 2.5 and P < 0.05.

    Journal: EBioMedicine

    Article Title: LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis

    doi: 10.1016/j.ebiom.2019.11.024

    Figure Lengend Snippet: Expression Profiles of differently expressed lncRNAs and mRNAs screened by microarray analysis of fibroblast-like synoviocytes (FLSs) samples from rheumatoid arthritis (RA) group ( n = 3) and control group ( n = 3). With the threshold of fold changes > 1.5 and P < 0.05, (a) the Volcano Plot showed differentially expressed lncRNAs between RA-FLSs and HC-FLSs and (b) the Scatter Plot showed differentially expressed mRNAs. (c) The Heat Map showing distinguishable lncRNA expression profiles with fold changes > 2.5 and P < 0.05.

    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications.

    Techniques: Expressing, Microarray

    Verification of lncRNAs and effect of lncRNA PICSAR suppression on the proliferation of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). (a) Quantitative real-time reverse transcription PCR (qRT-PCR) verified 5 selected lncRNAs with the highest up-regulated differential expression according to the microarray analysis. LncRNA PICSAR is the most significantly up-regulated in RA-FLSs. (b) LncRNA PICSAR was also over-expressed in the synovial fluid of RA patients compared with the control group by qPCR. (c) The inhibition efficiency of specific PICSAR small interference RNA (siRNA) in RA-FLSs was detected by qRT-PCR. (d) Compared with negative control, the expression of p-ERK1/2 in the total ERK1/2 protein was significantly decreased in the RA-FLSs after transfection. (e) PICSAR knockdown in RA-FLSs decreases cell proliferation assessed by a Cell Counting Kit-8 assay. The absorbance at 450 nm wavelength of PICSAR-siRNA group were significantly reduced at the indicated time points compared to the other two control groups. All the results were presented as the mean ± standard deviation (S.D.) based on ≥ 3 replicates involving ≥ 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus control groups.

    Journal: EBioMedicine

    Article Title: LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis

    doi: 10.1016/j.ebiom.2019.11.024

    Figure Lengend Snippet: Verification of lncRNAs and effect of lncRNA PICSAR suppression on the proliferation of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLSs). (a) Quantitative real-time reverse transcription PCR (qRT-PCR) verified 5 selected lncRNAs with the highest up-regulated differential expression according to the microarray analysis. LncRNA PICSAR is the most significantly up-regulated in RA-FLSs. (b) LncRNA PICSAR was also over-expressed in the synovial fluid of RA patients compared with the control group by qPCR. (c) The inhibition efficiency of specific PICSAR small interference RNA (siRNA) in RA-FLSs was detected by qRT-PCR. (d) Compared with negative control, the expression of p-ERK1/2 in the total ERK1/2 protein was significantly decreased in the RA-FLSs after transfection. (e) PICSAR knockdown in RA-FLSs decreases cell proliferation assessed by a Cell Counting Kit-8 assay. The absorbance at 450 nm wavelength of PICSAR-siRNA group were significantly reduced at the indicated time points compared to the other two control groups. All the results were presented as the mean ± standard deviation (S.D.) based on ≥ 3 replicates involving ≥ 3 samples. * P < 0.05, ** P < 0.01, and *** P < 0.001 versus control groups.

    Article Snippet: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications.

    Techniques: Quantitative RT-PCR, Expressing, Microarray, Inhibition, Negative Control, Transfection, Cell Counting, Standard Deviation